Toxicity mechanism of titanium dioxide and zinc oxide nanoparticles against food pathogens

G Devanand Venkatasubbu; R Baskar; T Anusuya; C Arun Seshan; Ramachandran Chelliah

doi:10.1016/j.colsurfb.2016.09.042

Toxicity mechanism of titanium dioxide and zinc oxide nanoparticles against food pathogens

Colloids Surf B Biointerfaces. 2016 Dec 1:148:600-606. doi: 10.1016/j.colsurfb.2016.09.042. Epub 2016 Sep 28.

Authors

G Devanand Venkatasubbu¹, R Baskar², T Anusuya³, C Arun Seshan⁴, Ramachandran Chelliah⁵

Affiliations

¹ Department of Nanotechnology, SRM University, Kattankulathur, Tamil Nadu, India. Electronic address: gdevanandvenkatasubbu@gmail.com.
² Department of Biotechnology, University of Madras, Chennai, Tamil Nadu, India.
³ Department of Nanotechnology, SRM University, Kattankulathur, Tamil Nadu, India.
⁴ Crystal Growth Centre, Anna University, Chennai, Tamil Nadu, India.
⁵ Centre for Food Technology, Anna University, Chennai, Tamil Nadu, India.

PMID: 27694049
DOI: 10.1016/j.colsurfb.2016.09.042

Abstract

Food preservation is an important field of research. It extends the shelf life of major food products. Our current study is based on food preservation through TiO₂ and ZnO nanoparticles. TiO₂ and ZnO are biocompatible nanomaterial. The biocompatibility of the materials were established through toxicity studies on cell lines. Titanium dioxide and Zinc Oxide nanoparticle were synthesized by wet chemical process. They are characterized by X-Ray diffraction and TEM. The antibacterial activities of both the materials were analysed to ensure their effectiveness as food preservative against Salmonella typhi, Klebsiella pneumoniae and Shigella flexneri. The results indicates that TiO₂ and ZnO nanoparticle inhibits Salmonella, Klebsiella and Shigella. The mode of action is by the generation of ROS in cases of Salmonella, Klebsiella. Mode of action in Shigella is still unclear. It was also proved that TiO₂ and ZnO nanoparticle are biocompatible materials.

Keywords: Food pathogens; Klebsiella; Salmonella; Shigella flexneri; Titanium dioxide; Zinc oxide.

MeSH terms

Anti-Bacterial Agents / chemistry*
Anti-Bacterial Agents / toxicity
Biocompatible Materials / chemistry
Biocompatible Materials / pharmacology
Biocompatible Materials / toxicity
Cell Line, Tumor
Cell Survival / drug effects
DNA Fragmentation / drug effects
Food Microbiology
Food Preservatives / chemistry*
Food Preservatives / pharmacology
Food Preservatives / toxicity
Humans
Klebsiella pneumoniae / drug effects
Klebsiella pneumoniae / genetics
Klebsiella pneumoniae / growth & development
Metal Nanoparticles / chemistry*
Metal Nanoparticles / toxicity
Metal Nanoparticles / ultrastructure
Microscopy, Electron, Transmission
Nanostructures / chemistry
Nanostructures / toxicity
Nanostructures / ultrastructure
Reactive Oxygen Species / chemistry
Reactive Oxygen Species / metabolism
Salmonella typhi / drug effects
Salmonella typhi / genetics
Salmonella typhi / growth & development
Shigella flexneri / drug effects
Shigella flexneri / genetics
Shigella flexneri / growth & development
Titanium / chemistry*
X-Ray Diffraction
Zinc Oxide / chemistry*

Substances

Anti-Bacterial Agents
Biocompatible Materials
Food Preservatives
Reactive Oxygen Species
titanium dioxide
Titanium
Zinc Oxide