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Biochemistry. 1989 May 16;28(10):4422-6.

Flash photolysis of the serum albumin-heme-CO complex.

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  • 1INSERM U299, Hôpital de Bicêtre, Le Kremlin Bicêtre, France.


Protoheme-CO in aqueous solution does not exhibit a geminate ligand recombination reaction. Addition of a protein, either globin or serum albumin, to which heme binds strongly, leads to an observable geminate reaction in aqueous solution. The bimolecular kinetic data for the albumin-heme-CO complex show two stable components, one heme-like in rate and difference spectrum, and one hemoglobin-like. The geminate reaction correlates spectrally with the hemoglobin-like component.

[PubMed - indexed for MEDLINE]
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