Experimental investigation of the susceptibility of Italian Culex pipiens mosquitoes to Zika virus infection

Euro Surveill. 2016 Sep 1;21(35):30328. doi: 10.2807/1560-7917.ES.2016.21.35.30328.

Abstract

We investigated the susceptibility of an Italian population of Culex pipiens mosquitoes to Zika virus (ZIKV) infection, tested in parallel with Aedes aegypti, as a positive control. We analysed mosquitoes at 0, 3, 7, 10, 14, 20 and 24 days after an infectious blood meal. Viral RNA was detected in the body of Cx. pipiens up to three days post-infection, but not at later time points. Our results indicate that Cx. pipiens is not susceptible to ZIKV infection.

Keywords: Ae aegypti; Cx pipiens; Italy; Zika virus; experimental infection; vector competence.

MeSH terms

  • Aedes / virology*
  • Animals
  • Culex / virology*
  • Disease Susceptibility
  • Female
  • Insect Vectors / virology*
  • Real-Time Polymerase Chain Reaction
  • Viral Load
  • Zika Virus / genetics
  • Zika Virus / isolation & purification*
  • Zika Virus / pathogenicity*
  • Zika Virus Infection / transmission*
  • Zika Virus Infection / virology