J Biol Chem. 1989 Aug 15;264(23):13407-11.

cDNA-derived amino acid sequence of the 86-kDa subunit of the Ku antigen.

Source

Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030.

Abstract

The Ku antigen is a DNA-associated nuclear protein recognized by sera from patients with autoimmune diseases. It consists of two polypeptides of 86 and 70 kDa. cDNA clones encoding the 86-kDa subunit of the Ku antigen were isolated by probing lambda gt11 recombinant cDNA expression libraries with a monoclonal antibody specific for this protein. The amino acid sequence deduced from the cDNA comprises 732 amino acids and corresponds to a protein with molecular weight of 81.914. Nineteen residues at the NH2 terminus determined by protein sequencing corresponded to the sequence deduced from the cDNA. The predicted amino acid sequence contains a region with repeating leucine residues similar to the "leucine zipper" structure observed in the c-myc, v-myc, and c-fos oncogene products. The largest cDNA hybridized to 2.7- and 3.4-kilobase poly(A)+ mRNAs from HeLa cells. The cDNA clones expressed fusion proteins immunoreactive with the monoclonal antibody and sera from patients with autoimmune diseases.

PMID:: 2760028; [PubMed - indexed for MEDLINE]

Free full text

LinkOut - more resources

Full Text Sources

HighWire - PDF

Research Materials

Supplemental Content

Save items

Related citations in PubMed

Isolation and characterization of cDNA encoding the 80-kDa subunit protein of the human autoantigen Ku (p70/p80) recognized by autoantibodies from patients with scleroderma-polymyositis overlap syndrome. [Proc Natl Acad Sci U S A. 1990]
Isolation and characterization of cDNA encoding the 80-kDa subunit protein of the human autoantigen Ku (p70/p80) recognized by autoantibodies from patients with scleroderma-polymyositis overlap syndrome.
Mimori T, Ohosone Y, Hama N, Suwa A, Akizuki M, Homma M, Griffith AJ, Hardin JA. Proc Natl Acad Sci U S A. 1990 Mar; 87(5):1777-81.
Molecular cloning of cDNA encoding the p70 (Ku) lupus autoantigen. [J Biol Chem. 1989]
Molecular cloning of cDNA encoding the p70 (Ku) lupus autoantigen.
Reeves WH, Sthoeger ZM. J Biol Chem. 1989 Mar 25; 264(9):5047-52.
Antigenic determinants of the 70-kDa subunit of the Ku autoantigen. [Clin Immunol Immunopathol. 1992]
Antigenic determinants of the 70-kDa subunit of the Ku autoantigen.
Abu-Elheiga L, Yaneva M. Clin Immunol Immunopathol. 1992 Aug; 64(2):145-52.
Human protein NEFA, a novel DNA binding/EF-hand/leucine zipper protein. Molecular cloning and sequence analysis of the cDNA, isolation and characterization of the protein. [Biol Chem Hoppe Seyler. 1994]
Human protein NEFA, a novel DNA binding/EF-hand/leucine zipper protein. Molecular cloning and sequence analysis of the cDNA, isolation and characterization of the protein.
Barnikol-Watanabe S, Gross NA, Götz H, Henkel T, Karabinos A, Kratzin H, Barnikol HU, Hilschmann N. Biol Chem Hoppe Seyler. 1994 Aug; 375(8):497-512.
The deduced protein sequence of the human carboxypeptidase N high molecular weight subunit reveals the presence of leucine-rich tandem repeats. [J Biol Chem. 1990]
The deduced protein sequence of the human carboxypeptidase N high molecular weight subunit reveals the presence of leucine-rich tandem repeats.
Tan F, Weerasinghe DK, Skidgel RA, Tamei H, Kaul RK, Roninson IB, Schilling JW, Erdös EG. J Biol Chem. 1990 Jan 5; 265(1):13-9.

See reviews... See all...

Cited by 30 PubMed Central articles

Non-homologous end joining, but not homologous recombination, enables survival for cells exposed to a histone deacetylase inhibitor. [Nucleic Acids Res. 2005]
Non-homologous end joining, but not homologous recombination, enables survival for cells exposed to a histone deacetylase inhibitor.
Yaneva M, Li H, Marple T, Hasty P. Nucleic Acids Res. 2005; 33(16):5320-30. Epub 2005 Sep 21.
The C terminus of Ku80 activates the DNA-dependent protein kinase catalytic subunit. [Mol Cell Biol. 1999]
The C terminus of Ku80 activates the DNA-dependent protein kinase catalytic subunit.
Singleton BK, Torres-Arzayus MI, Rottinghaus ST, Taccioli GE, Jeggo PA. Mol Cell Biol. 1999 May; 19(5):3267-77.
High affinity cross-reacting mAb generated by minimal mimicry: implications for the pathogenesis of anti-nuclear autoantibodies and immunosuppression. [Proc Natl Acad Sci U S A. 1998]
High affinity cross-reacting mAb generated by minimal mimicry: implications for the pathogenesis of anti-nuclear autoantibodies and immunosuppression.
Rothermel AL, Altieri DC. Proc Natl Acad Sci U S A. 1998 Mar 31; 95(7):3816-20.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound
PubChem chemical compound records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records. Multiple substance records may contribute to the PubChem compound record.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Gene
Gene records that cite the current articles. Citations in Gene are added manually by NCBI or imported from outside public resources.
HomoloGene
HomoloGene clusters of homologous genes and sequences that cite the current articles. These are references on the Gene and sequence records in the HomoloGene entry.
Nucleotide
Primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Nucleotide (RefSeq)
NCBI nucleotide Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Nucleotide (Weighted)
Nucleotide records associated with the current articles through the Gene database. These are the related sequences on the Gene record that are added manually by NCBI.
Protein (RefSeq)
NCBI protein Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Protein (Weighted)
Protein records associated with the current articles through related Gene database records. These are the related sequences on the Gene record that are added manually by NCBI.
Substance
PubChem chemical substance records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
UniGene
UniGene clusters of expressed sequences that are associated with the current articles through references on the clustered sequence records and related Gene records.
Protein
Protein translation features of primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
GEO Profiles
Gene Expression Omnibus (GEO) Profiles of molecular abundance data. The current articles are references on the Gene record associated with the GEO profile.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

cDNA-derived amino acid sequence of the 86-kDa subunit of the Ku antigen.
cDNA-derived amino acid sequence of the 86-kDa subunit of the Ku antigen.
J Biol Chem. 1989 Aug 15 ;264(23):13407-11.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

cDNA-derived amino acid sequence of the 86-kDa subunit of the Ku antigen.

Source

Abstract

Publication Types, MeSH Terms, Substances, Secondary Source ID, Grant Support

Publication Types

MeSH Terms

Substances

Secondary Source ID

Grant Support

LinkOut - more resources

Full Text Sources

Research Materials

Supplemental Content

Save items

Related citations in PubMed

Cited by 30 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information