Abstract
Copper plays a key role in the modulation of cellular function, defence, and growth. Here we present InCCu1, a ratiometric fluorescent sensor for mitochondrial copper, which changes from red to blue emission in the presence of Cu(i). Employing this probe in microscopy and flow cytometry, we show that cisplatin-treated cells have an impaired ability to accumulate copper in the mitochondria.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents / pharmacology
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Biosensing Techniques / methods*
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Cell Survival / drug effects
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Cisplatin / pharmacology
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Copper / metabolism*
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Fluorescent Dyes / metabolism*
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HeLa Cells
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Humans
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Mitochondria / drug effects
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Mitochondria / metabolism*
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Spectrometry, Fluorescence / methods*
Substances
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Antineoplastic Agents
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Fluorescent Dyes
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Copper
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Cisplatin