J Mol Biol. 1989 May 20;207(2):455-7.

Crystal structure and stability of a DNA duplex containing A(anti).G(syn) base-pairs.

Brown T, Leonard GA, Booth ED, Chambers J.

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Department of Chemistry, University of Edinburgh, U.K.

Abstract

The synthetic dodecanucleotide d(CGCAAATTGGCG) has been analysed by single-crystal X-ray diffraction techniques and the structure refined to R = 0.16 and 2.25 A resolution, with the location of 94 solvent molecules. The sequence crystallizes as a full turn of a B-DNA helix with ten Watson-Crick base-pairs and two adenine-guanine mispairs. The analysis clearly shows that the mismatches are of the form A(anti).G(syn). Thermal denaturation studies indicate that the stability of the duplex is strongly pH dependent, with a maximum at pH 5.0, suggesting that the base-pair is stabilized by protonation. Three different arrangements have been observed for base-pairs between guanine and adenine and it is likely that A.G mismatch conformation is strongly influenced by dipole-dipole interactions with adjacent base-pairs.

PMID:: 2754734; [PubMed - indexed for MEDLINE]

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Structures reported by this article

Crystal Structure And Stability Of A Dna Duplex Containing A(Anti).G(Syn) Base-Pairs

PDB: 111D

Source: synthetic construct

Method: X-Ray Diffraction

Resolution: 2.25 Å

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