Objective: To investigate the role of Tim-3 gene expression in acute graft-versus-host disease (aGVHD) after allogeneic hematopoietic stem cell transplantation (allo-HSCT).
Methods: Patients after allo-HSCT were retrospectively analyzed, this cohort of patients were divided into different groups according to disease states (grade 0-Ⅰ aGVHD group, grade Ⅱ-Ⅳ aGVHD group, improved aGVHD group) and time periods (+14-+30 d, +31-+60 d, +61-+100 d) to compare PBMC Tim-3 mRNA expression and plasma IFN-γ, IL-2 concentrations among them.
Results: RT-PCR showed that in grade 0-Ⅰ aGVHD group, Tim-3 mRNA expression in patients +31-+60 d (7.24±2.79) was significantly higher than that in patients + 14-+ 30 d (4.60±1.66) and + 61-+ 100 d (3.86±1.36) (P<0.05). Tim-3 mRNA expressions of grade Ⅱ-Ⅳ aGVHD group in patients +14-+30 d, +31-+60 d, +61-+100 d were 9.54± 3.05, 10.14±3.28, 12.82±4.20, respectively, which in both +14-+30 d and +61-+100 d were significantly higher than that of grade 0-Ⅰ aGVHD and improved aGVHD groups (P<0.05). In patients +31-+60 d, Tim-3 expression of grade Ⅱ-Ⅳ aGVHD group was higher than improved aGVHD group (2.49±0.89), while no statistical difference when compared with grade 0-Ⅰ aGVHD group (7.24±2.79). In grade Ⅱ-Ⅳ aGVHD group, Tim-3 mRNA expression manifested no statistical difference among grades or organ involved (P>0.05). ELISA results showed that plasma IFN-γ and IL-2 concentrations were higher in grade Ⅱ-Ⅳ aGVHD group than of other groups, while no significant difference existed between grade 0-Ⅰ aGVHD and improved aGVHD groups (P<0.05).
Conclusion: Tim-3 played an important role in the process of aGVHD.
目的: 探讨T细胞免疫球蛋白黏蛋白结构域分子3(Tim-3）mRNA表达在异基因造血干细胞移植（allo-HSCT）后急性移植物抗宿主病（aGVHD）中的作用。
方法: 回顾性分析100例allo-HSCT患者，根据患者aGVHD状态[0（未发生）~Ⅰ度aGVHD组、Ⅱ~Ⅳ度aGVHD治疗前组、Ⅱ~Ⅳ度aGVHD治疗好转组]及移植后不同阶段（+14~＋30 d、+31~＋60 d、+61~＋100 d）比较各组外周血单个核细胞Tim-3 mRNA及血浆IFN-γ、IL-2水平。
结果: 0~Ⅰ度aGVHD患者＋31~＋60 d Tim-3 mRNA相对定量（7.24±2.79）明显高于＋14~＋30 d(4.60±1.66）及＋61~＋100 d(3.86±1.36）（P值均<0.05）；+14~＋30 d、+31~＋60 d、+61~＋100 d Ⅱ~Ⅳ度aGVHD治疗前组的Tim-3 mRNA水平分别为9.54±3.05、10.14± 3.28、12.82±4.20，+14~+30 d、+61~＋100 d，均高于0~Ⅰ度aGVHD组及治疗好转组；+31~＋60 d Ⅱ~Ⅳ度aGVHD治疗前组明显高于治疗好转组（2.49±0.89），与0~Ⅰ度aGVHD组（7.24±2.79）相比差异无统计学意义；Ⅱ~Ⅳ度aGVHD组中Tim-3 mRNA水平在不同aGVHD程度、不同脏器之间比较差异均无统计学意义（P>0.05）。Ⅱ~Ⅳ度aGVHD治疗前组血浆IFN-γ、IL-2水平明显高于其他各组，治疗好转组血浆IFN-γ、IL-2水平与0~Ⅰ度aGVHD组比较差异无统计学意义。
结论: Tim-3在aGVHD的发生过程中发挥着重要作用。