Beyond "turn-on" readout: from zero background to signal amplification by combination of magnetic separation and plasmon enhanced fluorescence

Chem Commun (Camb). 2016 Aug 11;52(62):9660-3. doi: 10.1039/c6cc03850f. Epub 2016 Jul 11.

Abstract

By magnetic separation and subsequent plasmon enhanced fluorescence, an assay platform with a signal output from completely "zero" background to fluorescence amplification is achieved, using quantum dots as reporters. So, it well breaks through the conventional "turn-on" strategy in both lower and upper limits. The sensitivity for hyaluronidase sensing is enhanced 10(4)-10(6) times as compared with previous fluorescence methods.