J Biochem. 1989 Jan;105(1):51-4.

Identification of human apolipoprotein E variant gene: apolipoprotein E7 (Glu244,245----Lys244,245).

Maeda H, Nakamura H, Kobori S, Okada M, Mori H, Niki H, Ogura T, Hiraga S.

Source

Faculty of Health and Living Sciences, Naruto University of Education, Tokushima.

Abstract

Apolipoprotein E (apoE) is one of the protein moieties of the human serum lipoproteins. Three major isoforms of apoE (apoE2, apoE3, and apoE4) and minor variant isoforms (apoE1, apoE5, and apoE7) have been detected by isoelectric focusing. In this study we have cloned the apoE7 gene from a patient with the apoE3/E7 phenotype associated with hypertriglyceridemia and diabetes mellitus. DNA sequencing revealed that the apoE7 gene has two base substitutions (G----A) changing Glu244,245----Lys244,245, compared with the apoE3 gene. The replacement of the two amino acids is consistent with the result of isoelectric focusing of the apoE7 isoprotein, which shifts to four positively charged units compared with the apoE3 isoprotein.

PMID:: 2738044; [PubMed - indexed for MEDLINE]

Free full text

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Supplemental Content

Save items

Related citations in PubMed

Characterization of apolipoprotein E7 (Glu(244)-->Lys, Glu(245)--->Lys), a mutant apolipoprotein E associated with hyperlipidemia and atherosclerosis. [J Lipid Res. 1999]
Characterization of apolipoprotein E7 (Glu(244)-->Lys, Glu(245)--->Lys), a mutant apolipoprotein E associated with hyperlipidemia and atherosclerosis.
Yamamura T, Dong LM, Yamamoto A. J Lipid Res. 1999 Feb; 40(2):253-9.
Characterization of an apolipoprotein E3 variant (Arg 145-->His) associated with mild hypertriglyceridemia. [Ann Clin Lab Sci. 2001]
Characterization of an apolipoprotein E3 variant (Arg 145-->His) associated with mild hypertriglyceridemia.
Hidaka H, Tozuka M, Hidaka E, Yamauchi K, Ota H, Honda T, Katsuyama T. Ann Clin Lab Sci. 2001 Apr; 31(2):163-70.
Apolipoprotein E isoform phenotyping methodology and population frequency with identification of apoE1 and apoE5 isoforms. [J Lipid Res. 1987]
Apolipoprotein E isoform phenotyping methodology and population frequency with identification of apoE1 and apoE5 isoforms.
Ordovas JM, Litwack-Klein L, Wilson PW, Schaefer MM, Schaefer EJ. J Lipid Res. 1987 Apr; 28(4):371-80.
Review Apolipoprotein E isoforms in Alzheimer's disease pathology and etiology. [Microsc Res Tech. 2000]
Review Apolipoprotein E isoforms in Alzheimer's disease pathology and etiology.
Baum L, Chen L, Ng HK, Pang CP. Microsc Res Tech. 2000 Aug 15; 50(4):278-81.
Review Genetic heterogeneity of apolipoprotein E and its influence on plasma lipid and lipoprotein levels. [Hum Mutat. 1994]
Review Genetic heterogeneity of apolipoprotein E and its influence on plasma lipid and lipoprotein levels.
de Knijff P, van den Maagdenberg AM, Frants RR, Havekes LM. Hum Mutat. 1994; 4(3):178-94.

See reviews... See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Gene
Gene records that cite the current articles. Citations in Gene are added manually by NCBI or imported from outside public resources.
Gene (OMIM)
Gene records associated with Online Mendelian Inheritance in Man (OMIM) records that cite the current articles in their reference lists.
HomoloGene
HomoloGene clusters of homologous genes and sequences that cite the current articles. These are references on the Gene and sequence records in the HomoloGene entry.
Nucleotide (RefSeq)
NCBI nucleotide Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Nucleotide (Weighted)
Nucleotide records associated with the current articles through the Gene database. These are the related sequences on the Gene record that are added manually by NCBI.
OMIM (calculated)
Online Mendelian Inheritance in Man (OMIM) records that include the current articles as references in the light bulb links within or in the citations at the end of the OMIM record. The references available through the light bulb link are collected using the PubMed related articles algorithm to identify records with similar terminology to the OMIM record.
OMIM (cited)
Online Mendelian Inheritance in Man (OMIM) records that include the current articles as reference cited at the end of the OMIM record.
Protein (RefSeq)
NCBI protein Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Protein (Weighted)
Protein records associated with the current articles through related Gene database records. These are the related sequences on the Gene record that are added manually by NCBI.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
UniGene
UniGene clusters of expressed sequences that are associated with the current articles through references on the clustered sequence records and related Gene records.
GEO Profiles
Gene Expression Omnibus (GEO) Profiles of molecular abundance data. The current articles are references on the Gene record associated with the GEO profile.

Recent activity

Clear Turn Off Turn On

Identification of human apolipoprotein E variant gene: apolipoprotein E7 (Glu244...
Identification of human apolipoprotein E variant gene: apolipoprotein E7 (Glu244,245----Lys244,245).
J Biochem. 1989 Jan ;105(1):51-4.

PubMed
Adaptive and maladaptive functioning of institutionalized and noninstitutionaliz...
Adaptive and maladaptive functioning of institutionalized and noninstitutionalized fragile X males.
J Am Acad Child Adolesc Psychiatry. 1989 May ;28(3):427-30.

PubMed
[Planimetry of glaucomatous, para-papillary, chorio-pigment epithelial retinal a...
[Planimetry of glaucomatous, para-papillary, chorio-pigment epithelial retinal atrophy].
Fortschr Ophthalmol. 1989 ;86(2):92-4.

PubMed
Molecular characterization of beta-globin gene mutations in Malay patients with ...
Molecular characterization of beta-globin gene mutations in Malay patients with Hb E-beta-thalassaemia and thalassaemia major.
Br J Haematol. 1989 May ;72(1):73-80.

PubMed
Oestrogenic activity of tamoxifen and its metabolites on gene regulation and cel...
Oestrogenic activity of tamoxifen and its metabolites on gene regulation and cell proliferation in MCF-7 breast cancer cells.
Br J Cancer. 1989 May ;59(5):727-38.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: