Localization and characterization of the glycosylation site of human pancreatic elastase 1

P Wendorf; R Geyer; A Sziegoleit; D Linder

doi:10.1016/0014-5793(89)80640-4

Localization and characterization of the glycosylation site of human pancreatic elastase 1

FEBS Lett. 1989 Jun 5;249(2):275-8. doi: 10.1016/0014-5793(89)80640-4.

Authors

P Wendorf¹, R Geyer, A Sziegoleit, D Linder

Affiliation

¹ Institut für Medizinische Mikrobiologie der Universität, Giessen, FRG.

PMID: 2737288
DOI: 10.1016/0014-5793(89)80640-4

Abstract

Crystalline elastase 1 from human pancreas was digested with trypsin. Two peptides, containing the potential N-glycosylation sites at Asn-86 and Asn-125, were isolated and analyzed by amino acid analysis, sequencing and carbohydrate component analysis. The results demonstrate that only Asn-86 is glycosylated.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Amino Acids / analysis
Chromatography, Gel
Glycopeptides / isolation & purification
Glycosylation
Humans
Hydrolysis
Molecular Sequence Data
Pancreatic Elastase / metabolism*

Substances

Amino Acids
Glycopeptides
Pancreatic Elastase