Abstract
An improved organ culture system allowed to observe morphogenesis of mouse lingual papillae and taste buds relatively for longer period, in which fetal tongues were analyzed for 6 d. Taste cells were defined as eosinophobic epithelial cells expressing CK8 and Sox2 within lingual epithelium. Addition of glycogen synthase kinase 3 beta inhibitor CHIR99021 induced many taste cells and buds in non-gustatory and gustatory stratified lingual epithelium. The present study clearly demonstrated induction of taste cells and buds ectopically and without innervation.
Keywords:
Morphogenesis; Organ culture; Taste bud; β-catenin.
MeSH terms
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Animals
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Cell Nucleus / drug effects
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Cell Nucleus / metabolism
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Cells, Cultured
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Epithelium / drug effects
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Epithelium / innervation
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Fetus / physiology*
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Glycogen Synthase Kinase 3 beta / antagonists & inhibitors
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Glycogen Synthase Kinase 3 beta / metabolism
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Hedgehog Proteins / metabolism
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Mice, Inbred ICR
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Morphogenesis / drug effects
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Nerve Tissue / drug effects
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Nerve Tissue / physiology*
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Organ Culture Techniques / methods*
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Phospholipase C beta / metabolism
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Protein Kinase Inhibitors / pharmacology
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Protein Transport / drug effects
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Taste / drug effects
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Taste Buds / drug effects
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Taste Buds / embryology*
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Tongue / embryology*
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Wnt Signaling Pathway / drug effects
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beta Catenin / metabolism
Substances
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Hedgehog Proteins
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Protein Kinase Inhibitors
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Shh protein, mouse
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beta Catenin
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Glycogen Synthase Kinase 3 beta
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Phospholipase C beta