Derivation of Genea042 human embryonic stem cell line

Stem Cell Res. 2016 Mar;16(2):318-21. doi: 10.1016/j.scr.2015.11.011. Epub 2015 Nov 26.

Abstract

The Genea042 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, through ICM outgrowth on inactivated human feeders. The line showed pluripotent cell morphology and genomic analysis verified a 46, XX karyotype and female allele pattern through traditional karyotyping, CGH and STR analysis. Pluripotency of Genea042 was demonstrated with 81% of cells expressing Nanog, 95% Oct4, 53% Tra1-60 and 97% SSEA4, a PluriTest Pluripotency score of 30.06, Novelty score of 1.24 and Alkaline Phosphatase activity. The cell line was negative for Mycoplasma and any visible contamination.

MeSH terms

  • Blastocyst / cytology*
  • Cell Differentiation
  • Cells, Cultured
  • Cellular Reprogramming
  • Comparative Genomic Hybridization
  • Female
  • Human Embryonic Stem Cells / cytology*
  • Human Embryonic Stem Cells / metabolism
  • Humans
  • Karyotype
  • Microscopy, Fluorescence
  • Polymerase Chain Reaction
  • Transcription Factors / genetics
  • Transcription Factors / metabolism

Substances

  • Transcription Factors