CRISPR Outsourcing: Commissioning IHF for Site-Specific Integration of Foreign DNA at the CRISPR Array

Yunzhou Wei; Michael P Terns

doi:10.1016/j.molcel.2016.06.004

CRISPR Outsourcing: Commissioning IHF for Site-Specific Integration of Foreign DNA at the CRISPR Array

Mol Cell. 2016 Jun 16;62(6):803-804. doi: 10.1016/j.molcel.2016.06.004.

Authors

Yunzhou Wei¹, Michael P Terns²

Affiliations

¹ Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA.
² Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA; Department of Genetics, University of Georgia, Athens, GA 30602, USA; Department of Microbiology, University of Georgia, Athens, GA 30602, USA. Electronic address: mterns@uga.edu.

Abstract

In this issue of Molecular Cell, Nuñez et al. (2016) report that site-specific integration of foreign DNA into CRISPR loci by the Cas1-Cas2 integrase complex is promoted by a host factor, IHF (integration host factor), that binds and bends CRISPR leader DNA.

Publication types

Comment

MeSH terms

Bacterial Proteins / genetics
Base Sequence
CRISPR-Associated Proteins / genetics*
Clustered Regularly Interspaced Short Palindromic Repeats*
DNA
DNA-Binding Proteins / genetics
Escherichia coli / genetics
Integrases / genetics
Outsourced Services

Substances

Bacterial Proteins
CRISPR-Associated Proteins
DNA-Binding Proteins
DNA
Integrases

Abstract

Publication types

MeSH terms

Substances

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