P(VDF-TrFE)/BaTiO3 Nanoparticle Composite Films Mediate Piezoelectric Stimulation and Promote Differentiation of SH-SY5Y Neuroblastoma Cells

Adv Healthc Mater. 2016 Jul;5(14):1808-20. doi: 10.1002/adhm.201600245. Epub 2016 Jun 10.

Abstract

Poly(vinylidene fluoride-trifluoroethylene, P(VDF-TrFE)) and P(VDF-TrFE)/barium titanate nanoparticle (BTNP) films are prepared and tested as substrates for neuronal stimulation through direct piezoelectric effect. Films are characterized in terms of surface, mechanical, and piezoelectric features before in vitro testing on SH-SY5Y cells. In particular, BTNPs significantly improve piezoelectric properties of the films (4.5-fold increased d31 ). Both kinds of films support good SH-SY5Y viability and differentiation. Ultrasound (US) stimulation is proven to elicit Ca(2+) transients and to enhance differentiation in cells grown on the piezoelectric substrates. For the first time in the literature, this study demonstrates the suitability of polymer/ceramic composite films and US for neuronal stimulation through direct piezoelectric effect.

Keywords: BaTiO3 nanoparticles; P(VDF-TrFE); neurons; piezoelectric stimulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Barium Compounds / chemistry*
  • Cell Differentiation*
  • Cell Line, Tumor
  • Cell Survival
  • Electric Stimulation / methods
  • Humans
  • Polyvinyls / chemistry*
  • Titanium / chemistry*

Substances

  • Barium Compounds
  • Polyvinyls
  • barium titanate(IV)
  • vinylidene fluoride-trifluoroethylene copolymer
  • Titanium