Visualization of electrical field of electrode using voltage-controlled fluorescence release

Comput Biol Med. 2016 Aug 1:75:38-44. doi: 10.1016/j.compbiomed.2016.05.008. Epub 2016 May 16.

Abstract

In this study we propose an approach to directly visualize electrical current distribution at the electrode-electrolyte interface of a biopotential electrode. High-speed fluorescent microscopic images are acquired when an electric potential is applied across the interface to trigger the release of fluorescent material from the surface of the electrode. These images are analyzed computationally to obtain the distribution of the electric field from the fluorescent intensity of each pixel. Our approach allows direct observation of microscopic electrical current distribution around the electrode. Experiments are conducted to validate the feasibility of the fluorescent imaging method.

Keywords: Electrical current distribution; Electrode–electrolyte interface; Fluorescent tracer.

MeSH terms

  • Cell Line
  • Electrodes
  • Fluorescent Dyes / chemistry*
  • Humans
  • Image Processing, Computer-Assisted / methods*
  • Microscopy, Fluorescence

Substances

  • Fluorescent Dyes