Abstract
The antioxidant activity of methanolic extracts derived from shoots (HR-shoots) and roots (HR-roots) of pRi-transformed Rehmannia glutinosa plants were determined. The activity was indicated by the ability of the plant extracts to inhibit superoxide anion (O2(-·)) generation and thiobarbituric acid reactive substances (TBARS) production in resting blood platelets and platelets activated by thrombin. The strongest activity was exhibited by the HR-shoot extract (50 μg/mL). The present study also examines the antioxidant properties of the plant extracts against human plasma lipid peroxidation induced by strong biological oxidants: hydrogen peroxide (H2O2) and H2O2/Fe. The study shows that extracts from transformed R. glutinosa plants may be a promising source of natural antioxidants, which would be valuable in various cardiovascular diseases. The extracts may also protect lipids against oxidative modifications.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Antioxidants / chemistry
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Antioxidants / isolation & purification
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Antioxidants / pharmacology*
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Blood Platelets / drug effects
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Blood Platelets / metabolism
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Female
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Gene Expression Regulation, Plant
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Humans
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Hydrogen Peroxide / pharmacology
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Lipid Peroxidation / drug effects
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Male
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Methanol / chemistry*
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Oxidants / pharmacology
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Oxidative Stress / drug effects*
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Phytotherapy
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Plant Extracts / chemistry
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Plant Extracts / isolation & purification
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Plant Extracts / pharmacology*
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Plant Roots
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Plant Shoots
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Plants, Genetically Modified / chemistry
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Plants, Genetically Modified / genetics*
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Plants, Medicinal
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Rehmannia / chemistry
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Rehmannia / genetics*
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Solvents / chemistry*
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Superoxides / metabolism
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Thiobarbituric Acid Reactive Substances / metabolism
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Thrombin / pharmacology
Substances
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Antioxidants
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Oxidants
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Plant Extracts
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Solvents
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Thiobarbituric Acid Reactive Substances
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Superoxides
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Hydrogen Peroxide
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Thrombin
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Methanol