The optimal extraction conditions with a yield of 5.37±0.15% for extraction of polysaccharides from chickpea (Cicer arietinum L.) hull (CHPS) were determined as extraction temperature 99°C, extraction time 2.8h and ratio of water to raw material 24mL/g. Three fractions of CHPS-1, CHPS-2 and CHPS-3, with average molecular weight of 3.1×10(6), 1.5×10(6) and 7.8×10(5)Da, respectively, were obtained from crude CHPS by chromatography of DEAE Fast Flow and Sephadex G-100. CHPS-1 was composed of mannose, rhamnose, galactose, galacturonic acid, glucose and arabinose, CHPS-2 was composed of mannose, rhamnose, galacturonic acid, galactose, xylose and arabinose, CHPS-3 was composed of galacturonic acid, galactose and rhamnose. CHPS-3 showed the strongest reducing power and protective effect on H2O2-induced oxidative injury in PC12 cells and highest scavenging activities against DPPH and ABTS radicals, while CHPS-2 showed the highest scavenging activity against superoxide anion radical.
Keywords: 1,1-Diphenyl-2-picrylhydrazyl (PubChem CID: 74358); 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (PubChem CID: 64965); 3-Methyl-1-phenyl-2-pyrazolin-5-one (PubChem CID: 4021); Box-Behnken design; Chickpea hull; Cicer arietinum L; Hydrogen peroxide (PubChem CID: 784); In vitro antioxidant activity; Nitrotetrazolium blue chloride (PubChem CID: 9281); Polysaccharide extraction; Trifluoroacetic acid (PubChem CID: 6422); [2,2′-Azinobis-(3-ethyl-benzothiazolin-6-sulfonic acid)] diammonium salt (PubChem CID: 16240279).
Copyright © 2016 Elsevier Ltd. All rights reserved.