Generation and selection of monoclonal antibodies identifying surface molecules discriminating between fetal and adult fibroblasts

J Clin Lab Anal. 1989;3(1):50-5. doi: 10.1002/jcla.1860030110.

Abstract

The present report describes the reactivity of two murine monoclonal antibodies, i.e., CB11 and CB43, the first obtained by using embryonic material as immunizer and the second elicited against a human cell line. These reagents recognize two distinct molecules marking a peculiar differentiation stage on fetal/embryonic fibroblasts. The first structure is a single chain of 14.5 kd, the second one a heterodimer of 40-80 kd. Even if these reagents are not strictly fibroblast restricted, translineage expression does not diminish the medical relevance of the reagents; in fact, they may have application in prenatal diagnostics and amniocentesis testing, in which it is necessary to discriminate between fetal and maternal cells.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Amniocentesis
  • Antibodies, Monoclonal*
  • Antigens, Surface*
  • Cell Differentiation
  • Cell Line
  • Fetus / immunology
  • Fibroblasts / cytology
  • Fibroblasts / immunology*
  • Humans
  • Prenatal Diagnosis
  • Tumor Cells, Cultured / immunology

Substances

  • Antibodies, Monoclonal
  • Antigens, Surface