Indole-3-acetic acid-induced oxidative burst and an increase in cytosolic calcium ion concentration in rice suspension culture

Biosci Biotechnol Biochem. 2016 Aug;80(8):1546-54. doi: 10.1080/09168451.2016.1179094. Epub 2016 May 5.

Abstract

Indole-3-acetic acid (IAA) is the major natural auxin involved in the regulation of a variety of growth and developmental processes such as division, elongation, and polarity determination in growing plant cells. It has been shown that dividing and/or elongating plant cells accompanies the generation of reactive oxygen species (ROS) and a number of reports have suggested that hormonal actions can be mediated by ROS through ROS-mediated opening of ion channels. Here, we surveyed the link between the action of IAA, oxidative burst, and calcium channel activation in a transgenic cells of rice expressing aequorin in the cytosol. Application of IAA to the cells induced a rapid and transient generation of superoxide which was followed by a transient increase in cytosolic Ca(2+) concentration ([Ca(2+)]c). The IAA-induced [Ca(2+)]c elevation was inhibited by Ca(2+) channel blockers and a Ca(2+) chelator. Furthermore, ROS scavengers effectively blocked the action of IAA on [Ca(2+)]c elevation.

Keywords: Oryza sativa L; ROS; aequorin; auxin; calcium.

MeSH terms

  • Aequorin / genetics
  • Aequorin / metabolism
  • Calcium / metabolism*
  • Calcium Channel Blockers / pharmacology
  • Calcium Chelating Agents / pharmacology
  • Cations, Divalent
  • Cell Culture Techniques
  • Egtazic Acid / pharmacology
  • Gene Expression
  • Genes, Reporter
  • Indoleacetic Acids / pharmacology*
  • Ion Transport
  • Luminescent Measurements
  • Oryza / drug effects*
  • Oryza / genetics
  • Oryza / metabolism
  • Plant Cells / drug effects*
  • Plant Cells / metabolism
  • Plant Growth Regulators / pharmacology*
  • Plants, Genetically Modified
  • Reactive Oxygen Species / metabolism*
  • Respiratory Burst / drug effects
  • Signal Transduction
  • Verapamil / pharmacology

Substances

  • Calcium Channel Blockers
  • Calcium Chelating Agents
  • Cations, Divalent
  • Indoleacetic Acids
  • Plant Growth Regulators
  • Reactive Oxygen Species
  • Aequorin
  • Egtazic Acid
  • indoleacetic acid
  • Verapamil
  • Calcium