TP53, PIK3CA, FBXW7 and KRAS Mutations in Esophageal Cancer Identified by Targeted Sequencing

Cancer Genomics Proteomics. 2016 May-Jun;13(3):231-8.

Abstract

Background/aim: Esophageal cancer (EC) is a common malignancy with significant morbidity and mortality. As individual cancers exhibit unique mutation patterns, identifying and characterizing gene mutations in EC that may serve as biomarkers might help predict patient outcome and guide treatment. Traditionally, personalized cancer DNA sequencing was impractical and expensive. Recent technological advancements have made targeted DNA sequencing more cost- and time-effective with reliable results. This technology may be useful for clinicians to direct patient treatment.

Materials and methods: The Ion PGM and AmpliSeq Cancer Panel was used to identify mutations at 737 hotspot loci of 45 cancer-related genes in 64 EC samples from Chinese patients.

Results: Frequent mutations were found in TP53 and less frequent mutations in PIK3CA, FBXW7 and KRAS.

Conclusion: These results demonstrate that targeted sequencing can reliably identify mutations in individual tumors that make this technology a possibility for clinical use.

Keywords: Esophageal cancer; Ion PGM; genetic mutations; next-generation sequencing; personalized medicine; targeted therapy.

MeSH terms

  • Aged
  • Aged, 80 and over
  • Cell Cycle Proteins / genetics*
  • Class I Phosphatidylinositol 3-Kinases
  • Computational Biology
  • Databases, Genetic
  • Esophageal Neoplasms / genetics*
  • Esophageal Neoplasms / pathology
  • F-Box Proteins / genetics*
  • F-Box-WD Repeat-Containing Protein 7
  • Female
  • High-Throughput Nucleotide Sequencing
  • Humans
  • Male
  • Middle Aged
  • Mutation*
  • Neoplasm Grading
  • Phosphatidylinositol 3-Kinases / genetics*
  • Proto-Oncogene Proteins p21(ras) / genetics*
  • Reproducibility of Results
  • Tumor Suppressor Protein p53 / genetics*
  • Ubiquitin-Protein Ligases / genetics*

Substances

  • Cell Cycle Proteins
  • F-Box Proteins
  • F-Box-WD Repeat-Containing Protein 7
  • FBXW7 protein, human
  • KRAS protein, human
  • Tumor Suppressor Protein p53
  • Ubiquitin-Protein Ligases
  • Phosphatidylinositol 3-Kinases
  • Class I Phosphatidylinositol 3-Kinases
  • PIK3CA protein, human
  • Proto-Oncogene Proteins p21(ras)