Iron-Mediated Lysosomal Membrane Permeabilization in Ethanol-Induced Hepatic Oxidative Damage and Apoptosis: Protective Effects of Quercetin

Oxid Med Cell Longev. 2016:2016:4147610. doi: 10.1155/2016/4147610. Epub 2015 Dec 28.

Abstract

Iron, in its free ferrous states, can catalyze Fenton reaction to produce OH∙, which is recognized as a crucial role in the pathogenesis of alcoholic liver diseases (ALD). As a result of continuous decomposition of iron-containing compounds, lysosomes contain a pool of redox-active iron. To investigate the important role of intralysosomal iron in alcoholic liver injury and the potential protection of quercetin, male C57BL/6J mice fed by Lieber De Carli diets containing ethanol (30% of total calories) were cotreated by quercetin or deferoxamine (DFO) for 15 weeks and ethanol-incubated mice primary hepatocytes were pretreated with FeCl3, DFO, and bafilomycin A1 at their optimal concentrations and exposure times. Chronic ethanol consumption caused an evident increase in lysosomal redox-active iron accompanying sustained oxidative damage. Iron-mediated ROS could trigger lysosomal membrane permeabilization (LMP) and subsequent mitochondria apoptosis. The hepatotoxicity was attenuated by reducing lysosomal iron while being exacerbated by escalating lysosomal iron. Quercetin substantially alleviated the alcoholic liver oxidative damage and apoptosis by decreasing lysosome iron and ameliorating iron-mediated LMP, which provided a new prospective of the use of quercetin against ALD.

MeSH terms

  • Animals
  • Apoptosis / drug effects*
  • Body Weight / drug effects
  • Deferoxamine / pharmacology
  • Dietary Supplements
  • Ethanol / toxicity*
  • Feeding Behavior
  • Hepatocytes / drug effects
  • Hepatocytes / metabolism
  • Hepatocytes / pathology
  • Iron / pharmacology*
  • Liver / drug effects
  • Liver / pathology*
  • Lysosomes / drug effects
  • Lysosomes / metabolism*
  • Male
  • Membrane Potential, Mitochondrial / drug effects
  • Mice, Inbred C57BL
  • Oxidation-Reduction / drug effects
  • Oxidative Stress / drug effects*
  • Permeability
  • Protective Agents / pharmacology*
  • Quercetin / pharmacology*
  • Reactive Oxygen Species / metabolism

Substances

  • Protective Agents
  • Reactive Oxygen Species
  • Ethanol
  • Quercetin
  • Iron
  • Deferoxamine