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J Virol. 1989 May;63(5):1924-8.

Novel use of polymerase chain reaction to amplify cellular DNA adjacent to an integrated provirus.

Author information

  • 1Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.

Erratum in

  • J Virol 1990 Jun;64(6):3150.

Abstract

We describe a modification of the polymerase chain reaction technique which allows amplification of cellular DNA adjacent to an integrated provirus given sequence information for the provirus only. The modified technique should be generally useful for studies of insertional mutagenesis and other situations in which one wishes to isolate DNA adjacent to a region of known sequence.

PMID:
2704070
PMCID:
PMC250604
[PubMed - indexed for MEDLINE]
Free PMC Article
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