Enhancement of fructosyltransferase and fructooligosaccharides production by A. oryzae DIA-MF in Solid-State Fermentation using aguamiel as culture medium

Diana B Muñiz-Márquez; Juan C Contreras; Raúl Rodríguez; Solange I Mussatto; José A Teixeira; Cristóbal N Aguilar

doi:10.1016/j.biortech.2016.03.022

Enhancement of fructosyltransferase and fructooligosaccharides production by A. oryzae DIA-MF in Solid-State Fermentation using aguamiel as culture medium

Bioresour Technol. 2016 Aug:213:276-282. doi: 10.1016/j.biortech.2016.03.022. Epub 2016 Mar 19.

Authors

Diana B Muñiz-Márquez¹, Juan C Contreras¹, Raúl Rodríguez¹, Solange I Mussatto², José A Teixeira³, Cristóbal N Aguilar⁴

Affiliations

¹ Group of Bioprocesses, Food Research Department, School of Chemistry, University Autonomous of Coahuila, 25280 Saltillo, Coahuila, Mexico.
² Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, Netherlands.
³ IBB - Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus Gualtar, 4710-057 Braga, Portugal.
⁴ Group of Bioprocesses, Food Research Department, School of Chemistry, University Autonomous of Coahuila, 25280 Saltillo, Coahuila, Mexico. Electronic address: cristobal.aguilar@uadec.edu.mx.

PMID: 27036329
DOI: 10.1016/j.biortech.2016.03.022

Abstract

The aim of this work was to improve the production of fructosyltransferase (FTase) by Solid-State Fermentation (SSF) using aguamiel (agave sap) as culture medium and Aspergillus oryzae DIA-MF as producer strain. SSF was carried out evaluating the following parameters: inoculum rate, incubation temperature, initial pH and packing density to determine the most significant factors through Box-Hunter and Hunter design. The significant factors were then further optimized using a Box-Behnken design and response surface methodology. The maximum FTase activity (1347U/L) was obtained at 32°C, using packing density of 0.7g/cm(3). Inoculum rate and initial pH had no significant influence on the response. FOS synthesis applying the enzyme produced by A. oryzae DIA-MF was also studied using aguamiel as substrate.

Keywords: Aguamiel; Fructooligosaccharides; Fructosyltransferase; Solid State Fermentation.

MeSH terms

Aspergillus oryzae / enzymology
Aspergillus oryzae / metabolism*
Biotechnology / methods*
Culture Media
Fermentation
Hexosyltransferases / metabolism*
Hydrogen-Ion Concentration
Oligosaccharides / biosynthesis*
Temperature
Waste Products

Substances

Culture Media
Oligosaccharides
Waste Products
fructooligosaccharide
Hexosyltransferases
inulosucrase