DDX21 translocates from nucleus to cytoplasm and stimulates the innate immune response due to dengue virus infection

Biochem Biophys Res Commun. 2016 Apr 29;473(2):648-53. doi: 10.1016/j.bbrc.2016.03.120. Epub 2016 Mar 28.

Abstract

Successful DENV infection relies on its ability to evade the host innate immune system. By using iTRAQ labeling followed by LC-MS/MS analysis, DDX21 was identified as a new host RNA helicase involved in the DENV life cycle. In DENV infected cells, DDX21 translocates from nucleus to cytoplasm to active the innate immune response and thus inhibits DENV replication in the early stages of infection. DDX21 is then degraded by the viral NS2B-NS3 protease complex and the innate immunity is thus subverted to facilitate DENV replication. The results reveal a new mechanism in which DENV subverts the host innate immune system to facilitate its replication in host cells.

Keywords: DDX21; DENV; Innate immune response; NS2B/3 protease; iTRAQ.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • DEAD-box RNA Helicases / immunology*
  • DEAD-box RNA Helicases / metabolism
  • Dengue / immunology*
  • Dengue / metabolism
  • Dengue Virus / immunology*
  • Dengue Virus / physiology
  • Humans
  • Immunity, Innate*
  • Protein Transport
  • Proteolysis
  • Virus Replication

Substances

  • DDX21 protein, human
  • DEAD-box RNA Helicases