Further evidence from SSCP and ITS DNA sequencing support Trypanosoma evansi and Trypanosoma equiperdum as subspecies or even strains of Trypanosoma brucei

Yan-Zi Wen; Zhao-Rong Lun; Xing-Quan Zhu; Geoff Hide; De-Hua Lai

doi:10.1016/j.meegid.2016.03.022

Further evidence from SSCP and ITS DNA sequencing support Trypanosoma evansi and Trypanosoma equiperdum as subspecies or even strains of Trypanosoma brucei

Infect Genet Evol. 2016 Jul:41:56-62. doi: 10.1016/j.meegid.2016.03.022. Epub 2016 Mar 23.

Authors

Yan-Zi Wen¹, Zhao-Rong Lun², Xing-Quan Zhu³, Geoff Hide⁴, De-Hua Lai⁵

Affiliations

¹ Center for Parasitic Organisms, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China.
² Center for Parasitic Organisms, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China; State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China; Ecosystems & Environment Centre, School of Environment and Life Sciences, University of Salford, Salford M5 4WT, UK; Biomedical Research Centre, School of Environment and Life Sciences, University of Salford, Salford M5 4WT, UK.
³ State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China.
⁴ Ecosystems & Environment Centre, School of Environment and Life Sciences, University of Salford, Salford M5 4WT, UK; Biomedical Research Centre, School of Environment and Life Sciences, University of Salford, Salford M5 4WT, UK.
⁵ Center for Parasitic Organisms, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China. Electronic address: laidehua@mail.sysu.edu.cn.

PMID: 27016375
DOI: 10.1016/j.meegid.2016.03.022

Abstract

The subgenus Trypanozoon includes three species Trypanosoma brucei, Trypanosoma evansi and Trypanosoma equiperdum, which are morphologically identical and indistinguishable even using some molecular methods. In this study, PCR-based single strand conformation polymorphism (PCR-SSCP) was used to analyze the ribosomal DNA of the Trypanozoon species. Data indicate different patterns of ITS2 fragments between T. brucei, T. evansi and T. equiperdum by SSCP. Furthermore, analysis of total ITS sequences within these three members of the subgenus Trypanozoon showed a high degree of homology using phylogenetic analysis but were polyphyletic in haplotype networks. These data provide novel nuclear evidence to further support the notion that T. evansi and T. equiperdum should be subspecies or even strains of T. brucei.

Keywords: Haplotype network; ITS; PCR-SSCP; Phylogenetic tree; Trypanozoon.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Africa / epidemiology
Animals
Base Sequence
China / epidemiology
DNA, Protozoan / genetics*
DNA, Ribosomal / genetics*
Haplotypes
Humans
Insect Vectors / parasitology
Phylogeny*
Polymerase Chain Reaction
Polymorphism, Single-Stranded Conformational
Sequence Alignment
Sequence Homology, Nucleic Acid
Trypanosoma / classification*
Trypanosoma / genetics
Trypanosoma / isolation & purification
Trypanosoma brucei brucei / classification*
Trypanosoma brucei brucei / genetics
Trypanosoma brucei brucei / isolation & purification
Trypanosomiasis / epidemiology*
Trypanosomiasis / parasitology
Trypanosomiasis / transmission
Tsetse Flies / parasitology

Substances

DNA, Protozoan
DNA, Ribosomal