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Stain Technol. 1989 Sep;64(5):243-8.

A technique for fluorescence microscopy in semithin sections.

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  • 1Department of Anatomy and Cell Biology, Faculty of Medicine, University of Cantabria, Santander, Spain.


We describe here a procedure to improve contrast and resolution in fluorescence microscopy of sectioned tissues. Tissue fragments were fixed in ethanol-glacial acetic acid, embedded in diethylene glycol distearate, and semithin sectioned. This method maintains tissue antigenicity while preserving the structure of cells and tissues. The thinness of the sections eliminates scattered and emitted light from tissue structures outside the plane of focus. The procedure is simple and quick, and works excellently with fluorescein-conjugated lectins and antibodies.

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