Biotechniques. 1989 Mar;7(3):252-61.

Molecular cloning of sequence-specific DNA binding proteins using recognition site probes.

Source

Massachusetts Institute of Technology.

Abstract

Genes encoding sequence-specific DNA binding proteins can be isolated by screening lambda gt11 expression libraries with recognition site DNAs. This strategy is derived from that developed for the isolation of genes using antibody probes. Many different genes encoding transcriptional regulatory proteins have been cloned using this strategy. The DNA binding domains of these regulatory proteins contain different structural motifs including the helix-turn-helix, the "zinc finger" and the "leucine zipper". Various aspects of the screening strategy are evaluated and a detailed protocol is provided. In addition to binding site DNAs, protein and nucleotide probes have been successfully used to screen expression libraries. Therefore ligand based expression screening may be quite general in scope.

PMID:: 2698648; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

Supplemental Content

Save items

Related citations in PubMed

A nonradioactive screening method for cloning genes encoding sequence-specific DNA binding proteins. [Anal Biochem. 1991]
A nonradioactive screening method for cloning genes encoding sequence-specific DNA binding proteins.
Tan TH. Anal Biochem. 1991 Jan; 192(1):17-22.
Specific recognition site probes for isolating genes encoding DNA-binding proteins. [Methods Enzymol. 1993]
Specific recognition site probes for isolating genes encoding DNA-binding proteins.
Singh H. Methods Enzymol. 1993; 218:551-67.
Human protein NEFA, a novel DNA binding/EF-hand/leucine zipper protein. Molecular cloning and sequence analysis of the cDNA, isolation and characterization of the protein. [Biol Chem Hoppe Seyler. 1994]
Human protein NEFA, a novel DNA binding/EF-hand/leucine zipper protein. Molecular cloning and sequence analysis of the cDNA, isolation and characterization of the protein.
Barnikol-Watanabe S, Gross NA, Götz H, Henkel T, Karabinos A, Kratzin H, Barnikol HU, Hilschmann N. Biol Chem Hoppe Seyler. 1994 Aug; 375(8):497-512.
Review Cloning DNA binding proteins from cDNA expression libraries using oligonucleotide binding site probes. [Methods Mol Biol. 1994]
Review Cloning DNA binding proteins from cDNA expression libraries using oligonucleotide binding site probes.
Cowell IG, Hurst HC. Methods Mol Biol. 1994; 31:363-70.
Review DNA based diagnostic tests: recombinant DNA and cardiovascular disease risk factors. [Br Med Bull. 1990]
Review DNA based diagnostic tests: recombinant DNA and cardiovascular disease risk factors.
Sidoli A, Galliani S, Baralle FE. Br Med Bull. 1990 Oct; 46(4):941-59.

See reviews... See all...

Cited by 41 PubMed Central articles

Repression of aerobic leukotoxin transcription by integration host factor in Aggregatibacter actinomycetemcomitans. [Res Microbiol. 2010]
Repression of aerobic leukotoxin transcription by integration host factor in Aggregatibacter actinomycetemcomitans.
Kolodrubetz D, Phillips L, Burgum A. Res Microbiol. 2010 Sep; 161(7):541-8. Epub 2010 May 20.
Rational design of p53, an intrinsically unstructured protein, for the fabrication of novel molecular sensors. [J Biol Chem. 2005]
Rational design of p53, an intrinsically unstructured protein, for the fabrication of novel molecular sensors.
Geddie ML, O'Loughlin TL, Woods KK, Matsumura I. J Biol Chem. 2005 Oct 21; 280(42):35641-6. Epub 2005 Aug 23.
In vitro evolution of thermostable p53 variants. [Protein Sci. 1999]
In vitro evolution of thermostable p53 variants.
Matsumura I, Ellington AD. Protein Sci. 1999 Apr; 8(4):731-40.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound
PubChem chemical compound records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records. Multiple substance records may contribute to the PubChem compound record.
Substance
PubChem chemical substance records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Molecular cloning of sequence-specific DNA binding proteins using recognition si...
Molecular cloning of sequence-specific DNA binding proteins using recognition site probes.
Biotechniques. 1989 Mar ;7(3):252-61.

PubMed
Clinical value of ultrasonography in the detection and removal of radiolucent fo...
Clinical value of ultrasonography in the detection and removal of radiolucent foreign bodies.
Injury. 1989 Nov ;20(6):341-3.

PubMed
Improvement of Durie & Salmon staging for multiple myeloma by adding platelet co...
Improvement of Durie & Salmon staging for multiple myeloma by adding platelet count as a stratifying variable: a multivariate regression analysis of 163 untreated patients.
Eur J Haematol Suppl. 1989 ;51:99-104.

PubMed
Pathophysiology of malignant hyperthermia.
Pathophysiology of malignant hyperthermia.
Ann Fr Anesth Reanim. 1989 ;8(5):411-6.

PubMed
Werner syndrome.
Werner syndrome.
Aust N Z J Med. 1977 Jun ;7(3):309-11.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Molecular cloning of sequence-specific DNA binding proteins using recognition site probes.

Source

Abstract

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

Supplemental Content

Save items

Related citations in PubMed

Cited by 41 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information