MALDI-TOF mass spectrometry for differentiation between Streptococcus pneumoniae and Streptococcus pseudopneumoniae

Joffrey van Prehn; Suzanne Q van Veen; Jacqueline J G Schelfaut; Els Wessels

doi:10.1016/j.diagmicrobio.2016.01.012

MALDI-TOF mass spectrometry for differentiation between Streptococcus pneumoniae and Streptococcus pseudopneumoniae

Diagn Microbiol Infect Dis. 2016 May;85(1):9-11. doi: 10.1016/j.diagmicrobio.2016.01.012. Epub 2016 Jan 22.

Authors

Joffrey van Prehn¹, Suzanne Q van Veen², Jacqueline J G Schelfaut³, Els Wessels³

Affiliations

¹ Department of Medical Microbiology and Infection Control, VU University Medical Center, Amsterdam, The Netherlands; Department of Medical Microbiology, Onze Lieve Vrouwe Gasthuis, Amsterdam, The Netherlands. Electronic address: j.vanprehn@vumc.nl.
² Department of Medical Microbiology, Onze Lieve Vrouwe Gasthuis, Amsterdam, The Netherlands.
³ Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands.

PMID: 26971637
DOI: 10.1016/j.diagmicrobio.2016.01.012

Abstract

We compared the Vitek MS and Microflex MALDI-TOF mass spectrometry platform for species differentiation within the Streptococcus mitis group with PCR assays targeted at lytA, Spn9802, and recA as reference standard. The Vitek MS correctly identified 10/11 Streptococcus pneumoniae, 13/13 Streptococcus pseudopneumoniae, and 12/13 S. mitis/oralis. The Microflex correctly identified 9/11 S. pneumoniae, 0/13 S. pseudopneumoniae, and 13/13 S. mitis/oralis. MALDI-TOF is a powerful tool for species determination within the mitis group. Diagnostic accuracy varies depending on platform and database used.

Keywords: Diagnostic accuracy; MALDI-TOF; Mass spectrometry; Mitis group; PCR; Streptococcus.

MeSH terms

Humans
Molecular Typing
Sensitivity and Specificity
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Streptococcus / classification*
Streptococcus / genetics
Streptococcus pneumoniae / classification*
Streptococcus pneumoniae / genetics