Single step purification of concanavalin A (Con A) and bio-sugar production from jack bean using glucosylated magnetic nano matrix

Bioresour Technol. 2016 Aug:213:257-261. doi: 10.1016/j.biortech.2016.02.068. Epub 2016 Feb 23.

Abstract

Jack bean (JB, Canavalia ensiformis) is the source of bio-based products, such as proteins and bio-sugars that contribute to modern molecular biology and biomedical research. In this study, the use of jack bean was evaluated as a source for concanavalin A (Con A) and bio-sugar production. A novel method for purifying Con A from JBs was successfully developed using a glucosylated magnetic nano matrix (GMNM) as a physical support, which facilitated easy separation and purification of Con A. In addition, the enzymatic conversion rate of 2% (w/v) Con A extracted residue to bio-sugar was 98.4%. Therefore, this new approach for the production of Con A and bio-sugar is potentially useful for obtaining bio-based products from jack bean.

Keywords: Bio-sugar; Concanavalin A; Glucosylated magnetic nano matrix; Jack bean; Magnetic separation.

MeSH terms

  • Biotechnology / instrumentation
  • Biotechnology / methods*
  • Canavalia / metabolism*
  • Carbohydrates
  • Concanavalin A / isolation & purification*
  • Glycosylation
  • Monosaccharides / metabolism
  • Nanostructures

Substances

  • Carbohydrates
  • Monosaccharides
  • Concanavalin A