Estrogen Deficiency Leads to Further Bone Loss in the Mandible of CKD Mice

Yuchen Guo; Ningyuan Sun; Xiaobo Duan; Xin Xu; Liwei Zheng; Dutmanee Seriwatanachai; Yongyue Wang; Quan Yuan

doi:10.1371/journal.pone.0148804

Estrogen Deficiency Leads to Further Bone Loss in the Mandible of CKD Mice

PLoS One. 2016 Feb 17;11(2):e0148804. doi: 10.1371/journal.pone.0148804. eCollection 2016.

Authors

Yuchen Guo¹, Ningyuan Sun¹, Xiaobo Duan¹, Xin Xu¹, Liwei Zheng¹, Dutmanee Seriwatanachai², Yongyue Wang¹, Quan Yuan¹

Affiliations

¹ State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
² Department of Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.

Abstract

Background: Chronic kidney disease (CKD) has been regarded as a grave public health problem. Estrogen is a critical factor for both renal protection and bone remodeling. Our previous study demonstrated that CKD impairs the healing of titanium implants. The aim of this study was to investigate the effects of estrogen deficiency on the mandibular bone in CKD mice.

Methods: Forty eleven-week-old female C57BL mice were used in this study. Uremia and estrogen deficiency were induced by 5/6 nephrectomy and ovariectomy (OVX), respectively. After 8 weeks, the mice were sacrificed, and their mandibles were collected for micro-CT analysis and histological examination.

Results: All the mice survived the experimental period. Serum measurements confirmed a significant increase in BUN in the CKD group that was further increased by OVX. OVX led to significant decreases in both the BV/TV and cortical thickness of the mandibular bone in CKD mice.

Conclusion: In summary, our findings indicate that estrogen deficiency leads to further mandibular bone loss in CKD mice.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bone Resorption / pathology*
Estrogens / deficiency*
Estrogens / metabolism
Female
Mandible / diagnostic imaging
Mandible / pathology*
Mice, Inbred C57BL
Periodontal Ligament / diagnostic imaging
Periodontal Ligament / pathology
Renal Insufficiency, Chronic / blood
Renal Insufficiency, Chronic / diagnostic imaging
Staining and Labeling
X-Ray Microtomography

Substances

Estrogens

Grants and funding

This work was supported by grants from the National Natural Science Foundation of China (NSFC 81371173, 81321002) (http://www.nsfc.gov.cn/), 111 project of Ministry of Education of China, and Sichuan Province Youth Fund (2013JQ0017). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.