Infrared-active quadruple contrast FePt nanoparticles for multiple scale molecular imaging

Biomaterials. 2016 Apr:85:54-64. doi: 10.1016/j.biomaterials.2016.01.053. Epub 2016 Jan 27.

Abstract

A single nanomaterial with multiple imaging contrasts and functions is highly desired for multiscale theragnosis. Herein, we demonstrate single 1-1.9 μm infrared-active FePt alloy nanoparticles (FePt NPs) offering unprecedented four-contrast-in-one molecular imaging - computed tomography (CT), magnetic resonance imaging (MRI), photoacoustic (PA) imaging, and high-order multiphoton luminescence (HOMPL) microscopy. The PA response of FePt NPs outperforms that of infrared-active gold nanorods by 3- to 5.6-fold under identical excitation fluence and particle concentrations. HOMPL (680 nm) of an isolated FePt NP renders spatial full-width-at-half-maximum values of 432 nm and 300 nm beyond the optical diffraction limit for 1230-nm and 920-nm excitation, respectively. The in vivo targeting function was successfully visualized using HOMPL, PA imaging, CT, and MRI, thereby validating FePt as a single nanomaterial system covering up to four types (Optical/PA/CT/MRI) of molecular imaging contrast, ranging from the microscopic level to whole-body scale investigation.

Keywords: FePt nanoparticle; Infrared-active; Molecular imaging; Multi-modality contrast; Multiphoton luminescence; Photoacoustic responses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Contrast Media / chemistry*
  • Iron / chemistry*
  • Luminescence
  • Magnetic Resonance Imaging
  • Metal Nanoparticles / chemistry*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Molecular Imaging*
  • Nanotubes / chemistry
  • Photoacoustic Techniques
  • Platinum / chemistry*
  • Reproducibility of Results
  • Tomography, X-Ray Computed

Substances

  • Contrast Media
  • Platinum
  • Iron