Abstract
Post-translational modifications (PTMs) have key roles in regulating protein-protein interactions in living cells. However, it remains a challenge to identify these PTM-mediated interactions. Here we develop a new lysine-based photo-reactive amino acid, termed photo-lysine. We demonstrate that photo-lysine, which is readily incorporated into proteins by native mammalian translation machinery, can be used to capture and identify proteins that recognize lysine PTMs, including 'readers' and 'erasers' of histone modifications.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Click Chemistry
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Diazomethane / analogs & derivatives*
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Diazomethane / chemistry
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Diazomethane / metabolism
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HeLa Cells
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Histones / chemistry
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Histones / metabolism
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Humans
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Light*
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Lysine / analogs & derivatives*
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Lysine / chemistry
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Lysine / genetics
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Lysine / metabolism*
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Models, Molecular
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Molecular Structure
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Protein Binding
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Protein Processing, Post-Translational*
Substances
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Histones
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photo-lysine
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Diazomethane
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Lysine
Associated data
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PubChem-Substance/255366218
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PubChem-Substance/255366219
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PubChem-Substance/255366220
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PubChem-Substance/255366221
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PubChem-Substance/255366222
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PubChem-Substance/255366223
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PubChem-Substance/255366224
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PubChem-Substance/255366225
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PubChem-Substance/255366226
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PubChem-Substance/255366227
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PubChem-Substance/255366228
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PubChem-Substance/255366229
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PubChem-Substance/255366230
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PubChem-Substance/255366231
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PubChem-Substance/255366232
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PubChem-Substance/255366233
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PubChem-Substance/255366234
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PubChem-Substance/255366235
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PubChem-Substance/255366236
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PubChem-Substance/255366237
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PubChem-Substance/255366238
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PubChem-Substance/255366239
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PubChem-Substance/255366240
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PubChem-Substance/255366241
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PubChem-Substance/255366242
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PubChem-Substance/255366243