-
Dual role of interfacial phospholipid in phospholipase A2 catalysis.
The results of crosslinking experiments with dimethyl suberimidate and gel filtration binding studies are used to delineate a detailed model for phospholipase A2(phosphatide 2-acyl-hydrolase, EC 3.1.1.4) action in the presence of Ca2+ on mixed micelles of Triton X-100 and phospholipid. Important features of the "dual-phospholipid" model are: (i) the use of the nonionic surfactant as an inert matrix that may influence lipid conformation but does not interact with the enzyme; (ii) the involvement of two lipid molecules in a single cycle of catalysis as an explanation for the "surface dilution" phenomenon; (iii) the requirement of an ordered reaction whereby divalent metal ion binds prior to phospholipid binding; and (iv) the induction by lipid substrate of an asymmetric dimer structure for the enzyme.
PMID: 266715 [PubMed - indexed for MEDLINE]
PMCID: PMC431050
-
Cited by 8 PubMed Central articles
-
Interaction of group IA phospholipase A2 with metal ions and phospholipid vesicles probed with deuterium exchange mass spectrometry.
Burke JE, Karbarz MJ, Deems RA, Li S, Woods VL Jr, Dennis EA.
Biochemistry. 2008 Jun 17; 47(24):6451-9. Epub 2008 May 24.
[Biochemistry. 2008]
-
The role and characterization of phospholipase A1 in mediating lysophosphatidylcholine synthesis in Trypanosoma brucei.
Richmond GS, Smith TK.
Biochem J. 2007 Jul 15; 405(2):319-29.
[Biochem J. 2007]
-
Sequence-induced trimerization of phospholipase A2: structure of a trimeric isoform of PLA2 from common krait (Bungarus caeruleus) at 2.5 A resolution.
Singh G, Gourinath S, Saravanan K, Sharma S, Bhanumathi S, Betzel Ch, Srinivasan A, Singh TP.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jan 1; 61(Pt 1):8-13. Epub 2004 Oct 16.
[Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005]
- » See all...