Interactive Effects of Metal Mixtures on Seed Germination and Bioluminescence Activities based on the Theory of Probabilities

The interactive effects of metal mixtures on seed germination (36 combinations) and bacterial bioluminescence (60 combinations) activities were investigated based on the theory of probabilities. Observed effects were compared with expected effects, which were calculated based on single metal activities. Different responses and sensitivities were observed depending on the types and concentrations of mixture metals as well as the organisms tested. In general, an additive mode of action (observed and expected effects are nearly same) was mostly common in the bioluminescence assay, whereas synergistic and additive inhibition modes were mostly observed in seed germination. Therefore, the mixture effects of the different bioassays could not be generalized because they were dependent on the types and concentrations of chemicals and organisms used. The results indicated that mixture, rather than single, effects by various bioassays and wide ranges of concentration combinations may comprise a better strategy for the bioassessment of contaminated environments.