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Biomaterials. 2016 Jan;77:320-35. doi: 10.1016/j.biomaterials.2015.11.020. Epub 2015 Nov 14.

Orthotopic transplantation of a tissue engineered diaphragm in rats.

Author information

  • 1International Research, Clinical and Education Center of Regenerative Medicine, Kuban State Medical University, Krasnodar, Russian Federation.
  • 2Advanced Center for Translational Regenerative Medicine (ACTREM), Department of Clinical Science, Intervention and Technology (CLINTEC), Division of Ear, Nose and Throat, Karolinska Institutet, Stockholm, Sweden.
  • 3National Research Centre "Kurchatov Institute" (NRC "Kurchatov Institute") Laboratory of Polymer Materials, Moscow, Russian Federation.
  • 4Department of Human Physiology, Kuban State Medical University, Krasnodar, Russian Federation.
  • 5Common Use Center for Diagnostics of Nanomaterials, Structure and Properties, Kuban State University, Krasnodar, Russian Federation.
  • 6College of Medicine, University of Illinois, Chicago, IL, United States.
  • 7Department of Surgery, College of Medicine, University of Illinois, Chicago, IL, United States.
  • 8Texas Heart Institute, Center for Regenerative Medicine, Houston, TX, United States.
  • 9International Research, Clinical and Education Center of Regenerative Medicine, Kuban State Medical University, Krasnodar, Russian Federation; Advanced Center for Translational Regenerative Medicine (ACTREM), Department of Clinical Science, Intervention and Technology (CLINTEC), Division of Ear, Nose and Throat, Karolinska Institutet, Stockholm, Sweden. Electronic address: paolo.macchiarini@ki.se.

Abstract

The currently available surgical options to repair the diaphragm are associated with significant risks of defect recurrence, lack of growth potential and restored functionality. A tissue engineered diaphragm has the potential to improve surgical outcomes for patients with congenital or acquired disorders. Here we show that decellularized diaphragmatic tissue reseeded with bone marrow mesenchymal stromal cells (BM-MSCs) facilitates in situ regeneration of functional tissue. A novel bioreactor, using simultaneous perfusion and agitation, was used to rapidly decellularize rat diaphragms. The scaffolds retained architecture and mechanical properties and supported cell adhesion, proliferation and differentiation. Biocompatibility was further confirmed in vitro and in vivo. We replaced 80% of the left hemidiaphragm with reseeded diaphragmatic scaffolds. After three weeks, transplanted animals gained 32% weight, showed myography, spirometry parameters, and histological evaluations similar to native rats. In conclusion, our study suggested that reseeded decellularized diaphragmatic tissue appears to be a promising option for patients in need of diaphragmatic reconstruction.

Copyright © 2015 Elsevier Ltd. All rights reserved.

KEYWORDS:

Biocompatibility; Diaphragm; Scaffolds; Tissue engineering; Transplantation

PMID:
26618750
[PubMed - in process]
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