Format

Send to:

Choose Destination
See comment in PubMed Commons below
Mol Cell Biol. 1989 Jan;9(1):329-31.

A synthetic intron in a naturally intronless yeast pre-tRNA is spliced efficiently in vivo.

Author information

  • 1Laboratory of Genetics, University of Wisconsin, Madison 53706.

Abstract

Saccharomyces cerevisiae glutamine tRNA(CAG) is encoded by an intronless, single-copy gene, SUP60. We have imposed a requirement for splicing in the biosynthesis of this tRNA by inserting a synthetic intron in the SUP60 gene. Genetic analysis demonstrated that the interrupted gene produces a functional, mature tRNA product in vivo.

PMID:
2648132
[PubMed - indexed for MEDLINE]
PMCID:
PMC362177
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk