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Comput Biomed Res. 1989 Feb;22(1):52-62.

A new method for aligning histological serial sections for three-dimensional reconstruction.

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  • 1Zoologisches Institut der J. W. Goethe-Universität, Frankfurt am Main, Federal Republic of Germany.

Abstract

A new method for aligning histological serial sections for three-dimensional reconstruction by computer is introduced. This method is particularly suited for embedding structures in celloidine or paraplast. Prior to sectioning, at least three reference markers are affixed to the preparation in a direction perpendicular to the section plane, for easy identification on the finished sections. An algorithm translates and rotates the serial sections until all the reference markers are congruent. The main advantage of this method, however, is its capability to eliminate deformations caused by compression or stretching during sectioning by the determination of correction factors.

PMID:
2644065
[PubMed - indexed for MEDLINE]
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