Unraveling systematic inventory of Echinops (Asteraceae) with special reference to nrDNA ITS sequence-based molecular typing of Echinops abuzinadianus

Genet Mol Res. 2015 Oct 2;14(4):11752-62. doi: 10.4238/2015.October.2.9.

Abstract

The present study explored the systematic inventory of Echinops L. (Asteraceae) of Saudi Arabia, with special reference to the molecular typing of Echinops abuzinadianus Chaudhary, an endemic species to Saudi Arabia, based on the internal transcribed spacer (ITS) sequences (ITS1-5.8S-ITS2) of nuclear ribosomal DNA. A sequence similarity search using BLAST and a phylogenetic analysis of the ITS sequence of E. abuzinadianus revealed a high level of sequence similarity with E. glaberrimus DC. (section Ritropsis). The novel primary sequence and the secondary structure of ITS2 of E. abuzinadianus could potentially be used for molecular genotyping.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Asteraceae / classification
  • Asteraceae / genetics*
  • Base Sequence
  • DNA, Ribosomal Spacer / genetics*
  • Genotype*
  • Molecular Sequence Data
  • Molecular Typing / methods
  • Nucleic Acid Conformation
  • Phylogeny*
  • Saudi Arabia
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Species Specificity

Substances

  • DNA, Ribosomal Spacer