Proc Natl Acad Sci U S A. 1989 Jan;86(2):577-81.

Construction of functional artificial minichromosomes in the fission yeast Schizosaccharomyces pombe.

Hahnenberger KM, Baum MP, Polizzi CM, Carbon J, Clarke L.

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Department of Biological Sciences, University of California, Santa Barbara 93106.

Abstract

The centromere DNAs from chromosomes I and III of Schizosaccharomyces pombe have been cloned in an artificial chromosome vector in both budding and fission yeasts. In S. pombe, synthetic linear and circular minichromosomes containing an intact centromere are stable mitotically and behave as independent genetic linkage groups that segregate properly through meiosis. These experiments present a general strategy for the isolation of centromeres from other organisms.

PMID:: 2643117; [PubMed - indexed for MEDLINE]
PMCID:: PMC286515

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Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

MeSH Terms

Substances

DNA, Fungal

Grant Support

GM-33783/GM/NIGMS NIH HHS/United States

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