Host antioxidant enzymes and TLR-2 neutralization modulate intracellular survival of Staphylococcus aureus: Evidence of the effect of redox balance on host pathogen relationship during acute staphylococcal infection

Microb Pathog. 2015 Dec:89:114-27. doi: 10.1016/j.micpath.2015.09.007. Epub 2015 Sep 28.

Abstract

Staphylococcus aureus is an important pathogen in bone disease and innate immune recognition receptor, TLR-2 is reported to be crucial for inflammatory bone loss. Role of TLR-2 in bacterial clearance and cytokine response to S. aureus infection in murine bone marrow macrophages has been reported but the role of host derived ROS in host-pathogen relationship still remains an obvious question. In the present study, blocking of SOD and catalase in TLR-2 neutralized fresh bone marrow cells (FBMC) with Diethyldithiocarbamic acid (DDC) and 3-Amino-1,2,4-triazole (ATZ), separately, during acute S. aureus infection, produces moderate level of ROS and limits inflammation as compared with only TLR-2 non-neutralized condition and leads to decreased bacterial count compared with only TLR-2 neutralized condition. In summary, host SOD and catalase modulates ROS generation, cytokine levels and TLR-2 expression in FBMCs during acute S. aureus infection which might be useful in the alleviation of S. aureus infection and bone loss.

Keywords: Antioxidant inhibitors; Cytokine; Murine fresh bone marrow cells; S. aureus infection; Toll like receptor-2.

MeSH terms

  • Animals
  • Antioxidants / metabolism*
  • Catalase / metabolism*
  • Cells, Cultured
  • Disease Models, Animal
  • Host-Pathogen Interactions
  • Mice
  • Microbial Viability*
  • Oxidation-Reduction
  • Staphylococcal Infections / immunology*
  • Staphylococcus aureus / immunology*
  • Staphylococcus aureus / physiology
  • Superoxide Dismutase / metabolism*
  • Toll-Like Receptor 2 / metabolism*

Substances

  • Antioxidants
  • Tlr2 protein, mouse
  • Toll-Like Receptor 2
  • Catalase
  • Superoxide Dismutase