Mass spectrometry guided venom profiling and bioactivity screening of the Anatolian Meadow Viper, Vipera anatolica

Toxicon. 2015 Dec 1;107(Pt B):163-74. doi: 10.1016/j.toxicon.2015.09.013. Epub 2015 Sep 16.

Abstract

This contribution reports on the first characterization of the venom proteome and the bioactivity screening of Vipera anatolica, the Anatolian Meadow Viper. The crude venom as well as an isolated dimeric disintegrin showed remarkable cytotoxic activity against glioblastoma cells. Due to the rare occurrence and the small size of this species only little amount of venom was available, which was profiled by means of a combination of bottom-up and top-down mass spectrometry. From this analysis we identified snake venom metalloproteases, cysteine-rich secretory protein isoforms, a metalloprotease inhibitor, several type A2 phospholipases, disintegrins, a snake venom serine protease, a C-type lectin and a Kunitz-type protease inhibitor. Furthermore, we detected several isoforms of above mentioned proteins as well as previously unknown proteins, indicating an extensive complexity of the venom which would have remained undetected with conventional venomic approaches.

Keywords: Anatolian Meadow Viper; Bioactivity screening; Cytotoxicity; Snake venomics; Top-down venomics; Vipera anatolica; Viperidae.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Caco-2 Cells
  • Cell Line, Tumor
  • Cells, Cultured
  • Disintegrins / chemistry
  • Disintegrins / isolation & purification
  • Disintegrins / pharmacology
  • HEK293 Cells
  • HeLa Cells
  • Humans
  • Lectins, C-Type / chemistry
  • Lectins, C-Type / isolation & purification
  • MCF-7 Cells
  • Mass Spectrometry / methods*
  • Metalloproteases / antagonists & inhibitors
  • Metalloproteases / chemistry
  • Metalloproteases / isolation & purification
  • Metalloproteases / pharmacology
  • Phospholipases A2 / chemistry
  • Phospholipases A2 / isolation & purification
  • Phospholipases A2 / pharmacology
  • Protease Inhibitors / chemistry
  • Protease Inhibitors / isolation & purification
  • Protease Inhibitors / pharmacology
  • Protein Isoforms / chemistry
  • Protein Isoforms / isolation & purification
  • Protein Isoforms / pharmacology
  • Reptilian Proteins / antagonists & inhibitors
  • Reptilian Proteins / chemistry*
  • Reptilian Proteins / isolation & purification
  • Reptilian Proteins / pharmacology
  • Serine Proteases / chemistry
  • Serine Proteases / isolation & purification
  • Serine Proteases / pharmacology
  • Viper Venoms / chemistry*
  • Viperidae*

Substances

  • Disintegrins
  • Lectins, C-Type
  • Protease Inhibitors
  • Protein Isoforms
  • Reptilian Proteins
  • Viper Venoms
  • Phospholipases A2
  • Metalloproteases
  • Serine Proteases