Preparation of protoplasts of Ustilago violacea for transformation and pulse-field electrophoresis

Biotechniques. 1989 Sep;7(8):824, 826, 828.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Basidiomycota / cytology*
  • Cell Fractionation / methods*
  • Cellulase
  • Electrophoresis, Agar Gel
  • Enzymes
  • Glucuronidase
  • Magnesium Sulfate
  • Protoplasts*
  • Transformation, Genetic
  • Ustilago / cytology*

Substances

  • Enzymes
  • novozym 234
  • Magnesium Sulfate
  • Glucuronidase
  • Cellulase