A Novel 6'-N-Aminoglycoside Acetyltransferase, AAC(6')-Ial, from a Clinical Isolate of Serratia marcescens

Tatsuya Tada; Tohru Miyoshi-Akiyama; Kayo Shimada; Rajan K Dahal; Shyam K Mishra; Hiroshi Ohara; Teruo Kirikae; Bharat M Pokhrel

doi:10.1089/mdr.2015.0126

A Novel 6'-N-Aminoglycoside Acetyltransferase, AAC(6')-Ial, from a Clinical Isolate of Serratia marcescens

Microb Drug Resist. 2016 Mar;22(2):103-8. doi: 10.1089/mdr.2015.0126. Epub 2015 Aug 13.

Authors

Tatsuya Tada¹, Tohru Miyoshi-Akiyama², Kayo Shimada¹, Rajan K Dahal³, Shyam K Mishra³, Hiroshi Ohara⁴, Teruo Kirikae¹, Bharat M Pokhrel³

Affiliations

¹ 1 Department of Infectious Diseases, Research Institute , National Center for Global Health and Medicine, Tokyo, Japan .
² 2 Pathogenic Microbe Laboratory, Research Institute , National Center for Global Health and Medicine, Tokyo, Japan .
³ 3 Department of Microbiology, Institute of Medicine, Tribhuvan University , Kathmandu, Nepal .
⁴ 4 Department of International Medical-Cooperation, National Center for Global Health and Medicine , Tokyo, Japan .

PMID: 26270859
DOI: 10.1089/mdr.2015.0126

Abstract

Serratia marcescens IOMTU115 has a novel 6'-N-aminoglycoside acetyltransferase-encoding gene, aac(6')-Ial. The encoded protein AAC(6')-Ial has 146 amino acids, with 91.8% identity to the amino acid sequence of AAC(6')-Ic in S. marcescens SM16 and 97.3% identity to the amino acid sequence of AAC(6')-Iap in S. marcescens WW4. The minimum inhibitory concentrations of aminoglycosides for Escherichia coli expressing AAC(6')-Ial were similar to those for E. coli expressing AAC(6')-Ic or AAC(6')-Iap. Thin-layer chromatography showed that AAC(6')-Ial, AAC(6')-Ic, or AAC(6')-Iap acetylated all the aminoglycosides tested, except for apramycin, gentamicin, and lividomycin. Kinetics assays revealed that AAC(6')-Ial is a functional acetyltransferase against aminoglycosides. The aac(6')-Ial gene was located on chromosomal DNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acetylation
Acetyltransferases / genetics*
Acetyltransferases / metabolism
Amino Acid Sequence
Aminoglycosides / metabolism
Aminoglycosides / pharmacology*
Anti-Bacterial Agents / metabolism
Anti-Bacterial Agents / pharmacology*
Bacterial Proteins / genetics*
Bacterial Proteins / metabolism
Biotransformation
Chromosome Mapping
Cloning, Molecular
Escherichia coli / genetics
Escherichia coli / metabolism
Gene Expression
Humans
Microbial Sensitivity Tests
Open Reading Frames
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
Sequence Alignment
Serratia Infections / microbiology
Serratia marcescens / enzymology*
Serratia marcescens / genetics*
Serratia marcescens / isolation & purification

Substances

Aminoglycosides
Anti-Bacterial Agents
Bacterial Proteins
Recombinant Proteins
Acetyltransferases
aminoglycoside N(6')-acetyltransferase