Comparative study of multi-enzyme production from typical agro-industrial residues and ultrasound-assisted extraction of crude enzyme in fermentation with Aspergillus japonicus PJ01

Bioprocess Biosyst Eng. 2015 Oct;38(10):2013-22. doi: 10.1007/s00449-015-1442-3. Epub 2015 Aug 11.

Abstract

Submerged fermentation (SmF) and solid-state fermentation (SSF) of Aspergillus japonicus PJ01 for multi-enzyme complexes (MEC) production were comparatively studied. The results showed that orange peel and wheat bran were the best substrates for MEC production in SmF and SSF, respectively. After 72 h of cultivation under SmF, the maximal pectinase, CMCase, and xylanase activities reached 2610, 85, and 335 U/gds (units/gram dry substrate), respectively; while after 72 h of cultivation under SSF, these three enzymes' activities reached 966, 58, and 1004 U/gds, respectively. Effects of ultrasound on extraction of crude enzymes from SSF medium were determined, the maximal activities of pectinase, CMCase, and xylanase increased to 1.20, 1.48, and 1.30-fold, respectively. Apparent different mycelia growths of SSF and SmF were observed by scanning electron microscopy; and different isoforms of the crude enzyme extracts from SSF and SmF were presented by zymogram analysis.

Keywords: Multi-enzyme complexes; Solid-state fermentation; Submerged fermentation; Ultrasound; Zymography.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aspergillus / enzymology*
  • Aspergillus / growth & development
  • Aspergillus / radiation effects
  • Crops, Agricultural / microbiology*
  • Crops, Agricultural / radiation effects
  • Fermentation / physiology
  • Industrial Waste / prevention & control*
  • Multienzyme Complexes / biosynthesis*
  • Multienzyme Complexes / radiation effects
  • Sonication / methods*

Substances

  • Industrial Waste
  • Multienzyme Complexes