Comparative study of the radiobiological effects induced on adherent vs suspended cells by BNCT, neutrons and gamma rays treatments

L Cansolino; A M Clerici; C Zonta; P Dionigi; G Mazzini; R Di Liberto; S Altieri; F Ballarini; S Bortolussi; M P Carante; M Ferrari; S J González; I Postuma; N Protti; G A Santa Cruz; C Ferrari

doi:10.1016/j.apradiso.2015.07.054

Comparative study of the radiobiological effects induced on adherent vs suspended cells by BNCT, neutrons and gamma rays treatments

Appl Radiat Isot. 2015 Dec:106:226-32. doi: 10.1016/j.apradiso.2015.07.054. Epub 2015 Jul 30.

Authors

L Cansolino¹, A M Clerici², C Zonta², P Dionigi¹, G Mazzini³, R Di Liberto⁴, S Altieri⁵, F Ballarini⁵, S Bortolussi⁶, M P Carante⁵, M Ferrari⁵, S J González⁷, I Postuma⁵, N Protti⁵, G A Santa Cruz⁸, C Ferrari⁹

Affiliations

¹ Department of Clinic-Surgical Sciences, Experimental Surgery Laboratory, University of Pavia, Italy; IRCCS S. Matteo Hospital, Pavia, Italy.
² Department of Clinic-Surgical Sciences, Experimental Surgery Laboratory, University of Pavia, Italy.
³ IGM-CNR and Department of Biology and Biotechnologies "L. Spallanzani", University of Pavia, Italy.
⁴ IRCCS S. Matteo Hospital, Pavia, Italy.
⁵ Department of Physics, University of Pavia, Italy; INFN (National Institute of Nuclear Physics) Section of Pavia, Italy.
⁶ INFN (National Institute of Nuclear Physics) Section of Pavia, Italy.
⁷ Comisión Nacional de Energía Atómica (CNEA), Buenos Aires, Argentina; CONICET, Argentina.
⁸ Comisión Nacional de Energía Atómica (CNEA), Buenos Aires, Argentina.
⁹ Department of Clinic-Surgical Sciences, Experimental Surgery Laboratory, University of Pavia, Italy. Electronic address: cinzia.ferrari@unipv.it.

PMID: 26256647
DOI: 10.1016/j.apradiso.2015.07.054

Abstract

The present work is part of a preclinical in vitro study to assess the efficacy of BNCT applied to liver or lung coloncarcinoma metastases and to limb osteosarcoma. Adherent growing cell lines can be irradiated as adherent to the culture flasks or as cell suspensions, differences in radio-sensitivity of the two modalities of radiation exposure have been investigated. Dose related cell survival and cell cycle perturbation results evidenced that the radiosensitivity of adherent cells is higher than that of the suspended ones.

Keywords: BNCT; Cell cycle; Cell survival; Radiobiological effects.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Boron Neutron Capture Therapy*
Cell Adhesion / radiation effects*
Cell Cycle / radiation effects
Cell Line, Tumor
Gamma Rays*
Neoplasms / pathology
Neoplasms / radiotherapy*
Neutrons*
Rats