Extraction and elemental analysis of Coleus forskohlii extract

Pharmacognosy Res. 2015 Jul-Sep;7(3):237-41. doi: 10.4103/0974-8490.157966.

Abstract

Background: Coleus forskohlii Willd. is a popular traditional medicine used since ancient times for treatment of heart diseases, abdominal colic and respiratory disorders.

Objective: The aim of this study was to characterize the root extract of the medicinal plant Coleus forskohlii.

Materials and methods: Dry roots of C. forskohlii were used to extract Forskolin using toluene as a solvent. Thus, obtained extract of C. forskohlii was standardized to 30% and used for further studies.

Results: The physical properties of the extract were analyzed through scanning electron microscopy analysis, while the characterization of root extract through X-ray diffraction (XRD) and element analysis. The morphological feature of the C. forskohlii extract showed a flake like structure and the XRD showed sulfur trioxide (SO3) and trimer of sulfur trioxide (S3 O9). Through element analysis, elements such as carbon, oxygen, magnesium, aluminum, silicon, phosphorous, and sulfur were identified. Carbon showed the highest weight of 75.49% in comparison to all other elements.

Keywords: Coleus forskohlii; X-Ray diffraction; element analysis; physical properties; scanning electron microscope.