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Biol Chem Hoppe Seyler. 1989 Oct;370(10):1071-5.

Complete sequence of the coding region of human elongation factor 2 (EF-2) by enzymatic amplification of cDNA from human ovarian granulosa cells.

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  • 1Max-Planck-Institut für Biophysikalische Chemie, Abt. Molekulare Biologie, Göttingen.

Abstract

The use of two primers allowed the specific enzymatic amplification of elongation factor 2 starting with total double-stranded cDNA from human ovarian granulosa cells. The amplified DNA fragment with a length of 1765 bp was restricted and sequenced by the shot gun approach. From the sequences obtained from the amplified fragment and the cDNA insert of pHGR81 [Rapp et al. (1988) Biol. Chem. Hoppe-Seyler 369, 247-250] respectively, the DNA sequence containing the complete coding as well as the 3'-untranslated region was assembled.

PMID:
2610926
[PubMed - indexed for MEDLINE]
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