When viscous corrections to the inviscid acoustic radiation force theory are implemented and applied to a standing wave field, the direction of the acoustic radiation force on particles varies from theory to theory. Specifically, some theories predict that the direction of the force depends on the particle diameter, while others reveal that the direction of the force is independent of particle diameter. The present study is an experimental investigation of the direction of the acoustic radiation force which suggests that particle diameter does affect the direction. Experiments were conducted in air using an ultrasonic standing wave field with a nominal frequency of 30 kHz. Smoke particles and fine water droplets having a range of diameters were flowed into the region of a standing wave field. The direction of the acoustic radiation force was determined by observing whether the particles accumulated in the nodes or the anti-nodes of the standing wave. Results show a change in the direction of the acoustic radiation force at a particle diameter of 0.3±0.1 μm, which corresponds to a particle diameter to acoustic-boundary-layer thickness ratio of 0.023±0.008.