Production, quality control, and bio-distribution studies of (159)Gd-EDTMP as a palliative agent for bone pain

Simindokht Shirvani Arani; Somaye Ghasemi; Ali Bahrami Samani; Mojtaba Shamsaei Zafarghandi

doi:10.14661/2015.977-984

Production, quality control, and bio-distribution studies of (159)Gd-EDTMP as a palliative agent for bone pain

Electron Physician. 2015 Mar 1;7(1):977-84. doi: 10.14661/2015.977-984. eCollection 2015 Jan-Mar.

Authors

Simindokht Shirvani Arani¹, Somaye Ghasemi², Ali Bahrami Samani³, Mojtaba Shamsaei Zafarghandi⁴

Affiliations

¹ Assistant Professor, Radiopharmaceutical Research and Development Lab (RRDL), Nuclear Science and Technology Research Institute (NSTRI), Tehran, Iran.
² M.Sc. Student, Faculty of Nuclear Engineering and Physics, Amirkabir University of Technology, P. O. Box 15875-4413, Tehran, Iran.
³ Assistant Professor, Radiopharmaceutical Research and Development Lab (RRDL), Nuclear Science and Technology Research Institute (NSTRI), Tehran, P. O. Box: 14155-1339, Iran.
⁴ Associate Professor Faculty of Nuclear Engineering and Physics, Amirkabir University of Technology, P. O. Box 15875-4413, Tehran, Iran.

Abstract

Introduction: Particle-emitting, bone-seeking radiopharmaceuticals have attracted the attention of the nuclear medicine community over the last three decades for the treatment of the pain of osteoblastic metastases. The objectives of this research were to produce quality-controlled (159)Gd-EDTMP in order to provide a new therapeutic radiopharmaceutical for use in clinical applications.

Methods: The investigation was an experimental study in which (159)Gd (T1/2=18.479 h, Eβ (max)=970.60 keV, Eγ=363.55 (11.4%) keV] was produced by thermal neutron bombardment of natural Gd2O3 at the Tehran Research Reactor (TRR) for a period of 7 d at a flux of 3-4×10(13) neutrons/cm(2).s. It was then quality-controlled and used to radio-label the in-house prepared ethylene diamine tetra acetic acid (EDTM).

Results: Complexation parameters were optimized to achieve maximum yields (>99%). The radiochemical purity of (159)Gd-EDTMP was checked by radio thin layer chromatography RTLC. It was found to retain its stability at room temperature (>95%). Bio-distribution studies of the complexes conducted in wild rats showed significant bone uptake with rapid clearance from blood.

Conclusion: The properties of the (159)Gd-EDTMP that was produced suggest then use of a new, efficient, palliative therapeutic agent for metastatic bone pain instead of some other current radiopharmaceuticals.

Keywords: EDTMP; bone; gadolinium; radiotherapy.