Nanotechnologies in protein microarrays

Nanomedicine (Lond). 2015;10(17):2743-55. doi: 10.2217/nnm.15.81. Epub 2015 May 21.

Abstract

Protein microarray technology became an important research tool for study and detection of proteins, protein-protein interactions and a number of other applications. The utilization of nanoparticle-based materials and nanotechnology-based techniques for immobilization allows us not only to extend the surface for biomolecule immobilization resulting in enhanced substrate binding properties, decreased background signals and enhanced reporter systems for more sensitive assays. Generally in contemporarily developed microarray systems, multiple nanotechnology-based techniques are combined. In this review, applications of nanoparticles and nanotechnologies in creating protein microarrays, proteins immobilization and detection are summarized. We anticipate that advanced nanotechnologies can be exploited to expand promising fields of proteins identification, monitoring of protein-protein or drug-protein interactions, or proteins structures.

Keywords: DAPA; NAPPA; nanomaterials; quantum dots; recognition element.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Biomarkers / chemistry
  • DNA / chemistry
  • Drug Delivery Systems
  • Genes, Reporter
  • Gold / chemistry
  • Humans
  • Metal Nanoparticles / chemistry
  • Microscopy, Atomic Force
  • Nanoparticles / chemistry
  • Nanotechnology / methods*
  • Polysaccharides / chemistry
  • Protein Array Analysis / methods*
  • Protein Binding
  • Protein Interaction Mapping
  • Proteins / chemistry*
  • Surface Plasmon Resonance

Substances

  • Biomarkers
  • Polysaccharides
  • Proteins
  • Gold
  • DNA